Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Digestive tract

Cell type

Colorectal cancer organoid

NA

NA

Sample

source_name

Input DNA for REST ChIP-seq in context of shRNA knockdown of CDH1

tissue

CRC107 organoid

chip antibody

None

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Extraction of material for sequencing was performed as in http://dx.doi.org/10.1038/nature13736 Libraries were prepared according to Roche's instructions accompanying the kapa htp library prep kit, and DNA was amplified with 12 PCR cycles.

Sequencing Platform

instrument_model

Illumina HiSeq 4000

hg38

Number of total reads

40146863

Reads aligned (%)

97.4

Duplicates removed (%)

6.8

Number of peaks

900 (qval < 1E-05)

hg19

Number of total reads

40146863

Reads aligned (%)

96.8

Duplicates removed (%)

6.9

Number of peaks

725 (qval < 1E-05)