Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BEAF-32

Cell type

Cell type Class
Cell line
Cell type
S2
Source
Oregon R
Developmental Stage
late embryonic stage

Attributes by original data submitter

Sample

source_name
S2 cells
cell type
Late embryonic derived S2 cells
chip antibody
anti-Beaf32

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Standard chromatin extraction procedures were utilized for ChIP analysis using anti-Beaf32 or anti-IgG control antibodies. Antibodies were generated in the lab (Affinity purified, rabbit antibodies raised against 18AA-long C-terminal peptides. ChIP samples were prepared for sequencing using standard Illumina protocols of TruSeq ChIP sample prep kits from 1.2 ng of starting precipitated DNA to generate sequencing libraries

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

dm3

Number of total reads
5694512
Reads aligned (%)
63.6
Duplicates removed (%)
56.4
Number of peaks
5886 (qval < 1E-05)

dm6

Number of total reads
5694512
Reads aligned (%)
63.4
Duplicates removed (%)
57.0
Number of peaks
5963 (qval < 1E-05)

Base call quality data from DBCLS SRA