Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Embryonic fibroblast

Cell type

MEF

Tissue

Embryonic Fibroblast

Lineage

primaryCells

Description

Mouse Embryonic Fibroblast

Sample

source_name

lincRNA-p21+/+ murine MEF WCE input with Doxorubicin

strain background

hybrid 129/B6

genotype/variation

wild type: lincRNA-p21+/+

cell type

mouse embryonic fibroblast (MEF)

treatment

0.5 mM doxorubicin for 24hrs

chip antibody

none (input)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and bound DNA fragments were isolated with antibody. Genomic DNA libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

13871992

Reads aligned (%)

98.7

Duplicates removed (%)

11.2

Number of peaks

377 (qval < 1E-05)

mm9

Number of total reads

13871992

Reads aligned (%)

98.4

Duplicates removed (%)

11.4

Number of peaks

387 (qval < 1E-05)