Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MAFB

Cell type

Cell type Class
Epidermis
Cell type
Keratinocytes
MeSH Description
Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers of the epidermis to the cornified (horny) layer of the skin. Successive stages of differentiation of the keratinocytes forming the epidermal layers are basal cell, spinous or prickle cell, and the granular cell.

Attributes by original data submitter

Sample

source_name
human epidermal keratinocytes, MAFB ChIP
tissue
foreskin
cell type
primary human epidermal keratinocytes
chip antibody
MAFB (Santa Cruz, cat.# SC-7866, lot# G0312)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The isolated DNA was RNase treated and purified using Qiagen columns. We used the NEB Next ChIP-seq library prep kit; for sequencing libraries we followed Illumina's protocol.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
25685359
Reads aligned (%)
58.4
Duplicates removed (%)
7.7
Number of peaks
2170 (qval < 1E-05)

hg38

Number of total reads
25685359
Reads aligned (%)
59.6
Duplicates removed (%)
6.6
Number of peaks
2254 (qval < 1E-05)

Base call quality data from DBCLS SRA