Antigen

Antigen Class

TFs and others

Antigen

MAF

Cell type

Cell type Class

Epidermis

Cell type

Keratinocytes

MeSH Description

Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers of the epidermis to the cornified (horny) layer of the skin. Successive stages of differentiation of the keratinocytes forming the epidermal layers are basal cell, spinous or prickle cell, and the granular cell.

Sample

source_name

human epidermal keratinocytes, MAF ChIP

tissue

foreskin

cell type

primary human epidermal keratinocytes

chip antibody

MAF (Santa Cruz, cat.# SC-10022, lot# B1513)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

The isolated DNA was RNase treated and purified using Qiagen columns. We used the NEB Next ChIP-seq library prep kit; for sequencing libraries we followed Illumina's protocol.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

33975173

Reads aligned (%)

66.0

Duplicates removed (%)

6.9

Number of peaks

1551 (qval < 1E-05)

hg19

Number of total reads

33975173

Reads aligned (%)

65.5

Duplicates removed (%)

8.1

Number of peaks

1672 (qval < 1E-05)