Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
GFI1B

Cell type

Cell type Class
Blood
Cell type
CD34+
NA
NA

Attributes by original data submitter

Sample

source_name
Human primary adult proerythroblasts (ProEs)
cell type
CD34+ HSPC-derived proerythroblasts
tissue
bone marrow
developmental stage
adult
chip antibody
GFI1B - Abcam ab26132

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-seq analysis using the Illumin HiSeq2000, 10-20 ng of ChIP DNA was processed for library generation using the ChIP-seq Sample Preparation Kit (Illumina) following the manufacturer’s protocol. Raw ChIP-seq data were processed using the Illumina software pipeline. Only ChIP-seq reads that aligned to exactly one location in the reference human genome (UCSC, hg19) were retained for downstream data analysis.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
34334612
Reads aligned (%)
67.5
Duplicates removed (%)
30.8
Number of peaks
1990 (qval < 1E-05)

hg38

Number of total reads
34334612
Reads aligned (%)
69.5
Duplicates removed (%)
29.4
Number of peaks
2318 (qval < 1E-05)

Base call quality data from DBCLS SRA