Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Blood

Cell type

CD34+

NA

NA

Sample

source_name

Human primary adult proerythroblasts (ProEs)

cell type

CD34+ HSPC-derived proerythroblasts

tissue

bone marrow

developmental stage

adult

chip antibody

H3K27me3 - Millipore 07-449

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIP-seq analysis using the Illumin HiSeq2000, 10-20 ng of ChIP DNA was processed for library generation using the ChIP-seq Sample Preparation Kit (Illumina) following the manufacturer’s protocol. Raw ChIP-seq data were processed using the Illumina software pipeline. Only ChIP-seq reads that aligned to exactly one location in the reference human genome (UCSC, hg19) were retained for downstream data analysis.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

155656072

Reads aligned (%)

99.3

Duplicates removed (%)

43.9

Number of peaks

29643 (qval < 1E-05)

hg19

Number of total reads

155656072

Reads aligned (%)

99.0

Duplicates removed (%)

44.5

Number of peaks

29358 (qval < 1E-05)