GSM3070228: MDA-MB-231-1833 p27 ChIPSeq-rep1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
CDKN1B
Cell type
Cell type Class
Breast
Cell type
MDA-MB-231
Primary Tissue
Breast
Site of Extraction
Effusion, Pleural
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
MDA-MB-231-1833
cell line
derived from MDA-MB-231
cell type
cancer cells (a bone-tropic metastatic line)
tissue
breast
chip antibody
p27 (Cell Signaling; 3686S)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP assay was preformed by ChIP-IT High Sensitivity kit (Active Motif; 53040). After cells were crosslinked with 1% formaldehyde, the cells were lysed and sonicated. For the immunoprecipitation, sonicated samples were incubated with anti-cJun (Cell Signaling; 9165s) and p27 (Cell Signaling; 3686s) antibodies for overnight at 4°C. After elution DNA, the quality was measured using Bioanalyzer DNA HS (Agilent Technologies). Libraries were prepared using NEBNext® Ultra™ DNA Library Prep Kit (NEB), and their quality was measured by High Sensitivity D1000 Screen Tape (Agilent Technologies). ChIP sequencing was performed on Illumina 75 cycles 400M reads.