U937 cells containing empty expression vector, grown for 48 hours before harvest
cell line
U937
vector
empty expression vector
tissue
Established cell culture from histiocytic lymphoma
disease
histiocytic lymphoma
gender
male
age
37
antibody
Mouse-IgG
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Protein-DNA complexes were isolated from sonicated, crosslinked whole cell lysates using IgG mouse (IgG Control) or Flag antibodies. Crosslinking was then reversed by heating, and DNA was purified. Library preparation was performed by Dr. Louis Ramagli of the Sequencing and Microarray Facility (SMF) of MD Anderson Cancer center using the Beckman SPRIworks system.