Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Epitope tags

Cell type

Cell type Class
Blood
Cell type
U-937
Primary Tissue
Blood
Tissue Diagnosis
Lymphoma Diffuse Large B Cell

Attributes by original data submitter

Sample

source_name
U937 clone "1MIXL" containing MIXL1 enforced expression vector, grown for 48 hours before harvest
cell line
U937
vector
MIXL1 enforced expression vector
clone
1MIXL
tissue
Established cell culture from histiocytic lymphoma
disease
histiocytic lymphoma
gender
male
age
37
antibody
Flag (anti-Flag-M2, Sigma)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Protein-DNA complexes were isolated from sonicated, crosslinked whole cell lysates using IgG mouse (IgG Control) or Flag antibodies. Crosslinking was then reversed by heating, and DNA was purified. Library preparation was performed by Dr. Louis Ramagli of the Sequencing and Microarray Facility (SMF) of MD Anderson Cancer center using the Beckman SPRIworks system.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
11331191
Reads aligned (%)
95.5
Duplicates removed (%)
42.9
Number of peaks
1244 (qval < 1E-05)

hg19

Number of total reads
11331191
Reads aligned (%)
95.2
Duplicates removed (%)
43.2
Number of peaks
1330 (qval < 1E-05)

Base call quality data from DBCLS SRA