GSM3067897: H3K27ac ChIP-Seq UF 3 Input; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Cardiovascular
Cell type
HAEC
Primary Tissue
Aorta
Tissue Diagnosis
Normal
Attributes by original data submitter
Sample
source_name
Human Aortic Endothelial Cells
flow
Unidirectional Flow
passage
5-10
cell id
Lonza 3050605
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP, briefly, HAECs were fixed at room temperature with 1% paraformaldehyde in PBS for 10 min, and then quenched with glycine. Cells were collected by scraping. To generate sequencing libraries for ChIP-seq, DNA ends were repaired with T4 DNA Polymerase (Enzymatics #P7080L). Six-mer barcoded adapters (BIOO Scientific NEXTflex #514104) were ligated with T4 DNA Ligase (Enzymatics #L- 6030-HC- L). Libraries were then amplified less than 14 cycles by PCR (Phusion Hot Start II #F549L) and purified (Zymo #D5205) for high-throughput sequencing.