Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Cardiovascular
Cell type
HAEC
Primary Tissue
Aorta
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
Human Aortic Endothelial Cells
flow
Unidirectional Flow
passage
5-10
cell id
Lonza 3050605

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP, briefly, HAECs were fixed at room temperature with 1% paraformaldehyde in PBS for 10 min, and then quenched with glycine. Cells were collected by scraping. To generate sequencing libraries for ChIP-seq, DNA ends were repaired with T4 DNA Polymerase (Enzymatics #P7080L). Six-mer barcoded adapters (BIOO Scientific NEXTflex #514104) were ligated with T4 DNA Ligase (Enzymatics #L- 6030-HC- L). Libraries were then amplified less than 14 cycles by PCR (Phusion Hot Start II #F549L) and purified (Zymo #D5205) for high-throughput sequencing.

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg38

Number of total reads
22709605
Reads aligned (%)
98.8
Duplicates removed (%)
7.4
Number of peaks
984 (qval < 1E-05)

hg19

Number of total reads
22709605
Reads aligned (%)
97.6
Duplicates removed (%)
8.7
Number of peaks
813 (qval < 1E-05)

Base call quality data from DBCLS SRA