Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
Treg
NA
NA

Attributes by original data submitter

Sample

source_name
Effector memory TREG cells
disease state
T1D
individual
T26
tissue
Peripheral Blood
cell type
Effector memory TREG cells
chip antibody
H3K27ac (Abcam, ab4729)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared using ThruPLEX® DNA-seq Kit (Rubicon Genomics) according to the protocol from the vendor. Qubit, bioanalyzer and KAPA qPCR were done before pooling for sequencing on HiSeq 2500 platform.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
25942476
Reads aligned (%)
98.7
Duplicates removed (%)
5.2
Number of peaks
12675 (qval < 1E-05)

hg19

Number of total reads
25942476
Reads aligned (%)
98.1
Duplicates removed (%)
6.1
Number of peaks
12640 (qval < 1E-05)

Base call quality data from DBCLS SRA