GSM3067925: C14 Treg Input; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Blood
Cell type
Treg
NA
NA
Attributes by original data submitter
Sample
source_name
Effector memory TREG cells
disease state
control
individual
C14
tissue
Peripheral Blood
cell type
Effector memory TREG cells
chip antibody
None
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared using ThruPLEX® DNA-seq Kit (Rubicon Genomics) according to the protocol from the vendor. Qubit, bioanalyzer and KAPA qPCR were done before pooling for sequencing on HiSeq 2500 platform.