Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me1

Cell type

Cell type Class
Blood
Cell type
Treg
NA
NA

Attributes by original data submitter

Sample

source_name
Effector memory TREG cells
disease state
control
individual
C12
tissue
Peripheral Blood
cell type
Effector memory TREG cells
chip antibody
H3K4me1 (Abcam, ab8895)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared using ThruPLEX® DNA-seq Kit (Rubicon Genomics) according to the protocol from the vendor. Qubit, bioanalyzer and KAPA qPCR were done before pooling for sequencing on HiSeq 2500 platform.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
21130656
Reads aligned (%)
98.8
Duplicates removed (%)
3.7
Number of peaks
2767 (qval < 1E-05)

hg19

Number of total reads
21130656
Reads aligned (%)
98.2
Duplicates removed (%)
4.6
Number of peaks
2669 (qval < 1E-05)

Base call quality data from DBCLS SRA