Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
XBP1

Cell type

Cell type Class
Breast
Cell type
MDA-MB-231
Primary Tissue
Breast
Site of Extraction
Effusion, Pleural
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
breast cancer cells
cell type
triple negative breast cancer cells
chip antibody
XBP1 (Biolegend, 619502)
cell line
MDA-MB-231

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed as previously described by Chen et al. (Cell 2008). The ChIP-seq library was prepared using ChIP-Seq DNA Sample Prep Kit (Illumina) according to the manufacturer’s instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
209547103
Reads aligned (%)
56.1
Duplicates removed (%)
28.1
Number of peaks
3425 (qval < 1E-05)

hg38

Number of total reads
209547103
Reads aligned (%)
57.8
Duplicates removed (%)
26.5
Number of peaks
4252 (qval < 1E-05)

Base call quality data from DBCLS SRA