Antigen

Antigen Class

TFs and others

Antigen

XBP1

Cell type

Cell type Class

Breast

Cell type

MDA-MB-231

Primary Tissue

Breast

Site of Extraction

Effusion, Pleural

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

breast cancer cells

cell type

triple negative breast cancer cells

chip antibody

XBP1 (Biolegend, 619502)

cell line

MDA-MB-231

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP was performed as previously described by Chen et al. (Cell 2008). The ChIP-seq library was prepared using ChIP-Seq DNA Sample Prep Kit (Illumina) according to the manufacturer’s instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

209547103

Reads aligned (%)

78.7

Duplicates removed (%)

26.5

Number of peaks

4252 (qval < 1E-05)

hg19

Number of total reads

209547103

Reads aligned (%)

78.1

Duplicates removed (%)

28.1

Number of peaks

3425 (qval < 1E-05)