Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
PYGO2

Cell type

Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
Hela S3
chip antibody
Pygo2 (S3I4; Tzenov et al., J Clin Path.69, pp. 518-26, 2015 )

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates in RIPA buffer were clarified from sonicated nuclei and Pygo2-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina Truseq ChIP library preparation kit

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
54788788
Reads aligned (%)
83.3
Duplicates removed (%)
14.5
Number of peaks
16009 (qval < 1E-05)

hg38

Number of total reads
54788788
Reads aligned (%)
84.7
Duplicates removed (%)
13.5
Number of peaks
16407 (qval < 1E-05)

Base call quality data from DBCLS SRA