GSM1273657: ChIP-seq-AFG-input; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Pluripotent stem cell
Cell type
hESC derived mesendodermal cells
NA
NA
Attributes by original data submitter
Sample
source_name
hESC derived anterior foregut (AFG)
cell type
hESC derived anterior foregut (AFG)
chip antibody
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For Chip-seq, Lysates were clarified from sonicated cells and histone-DNA complexes were isolated with antibody. Antibodies were eluted from beads and formaldehyde cross-linking was reversed overnight by mild heating (65 °C), and chromatin was sequentially treated with RNase and Proteinase K before final column purification. Illumina sequencing libraries were generated using the TruSeq ChIP Sample Preparation Kit (Illumina). Briefly, 10 ng of ChIP-enriched DNA was end-repaired, 3’ adenylated, ligated with Illumina adapters, and amplified through 15 cycles of PCR amplification with Phusion High Fidelity DNA polymerase (Finnzymes) with primers directed against the adapters.