GSM1273644: ChIP-seq-H7-ESC-H3K4me3; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K4me3
Cell type
Cell type Class
Pluripotent stem cell
Cell type
hESC H7
NA
NA
Attributes by original data submitter
Sample
source_name
H7-Embryonic stem cell
cell type
H7 - Embryonic stem cell
chip antibody
H3K4me3 (abcam, ab8580)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For Chip-seq, Lysates were clarified from sonicated cells and histone-DNA complexes were isolated with antibody. Antibodies were eluted from beads and formaldehyde cross-linking was reversed overnight by mild heating (65 °C), and chromatin was sequentially treated with RNase and Proteinase K before final column purification. Illumina sequencing libraries were generated using the TruSeq ChIP Sample Preparation Kit (Illumina). Briefly, 10 ng of ChIP-enriched DNA was end-repaired, 3’ adenylated, ligated with Illumina adapters, and amplified through 15 cycles of PCR amplification with Phusion High Fidelity DNA polymerase (Finnzymes) with primers directed against the adapters.