Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Neural

Cell type

Neural Stem Cells

MeSH Description

Self-renewing cells that generate the main phenotypes of the nervous system in both the embryo and adult. Neural stem cells are precursors to both NEURONS and NEUROGLIA.

Sample

source_name

Neural stem cells

cell type

Neural stem cells

strain

C57BL/6

Sex

female

genotype

Smchd1 del/del Xist 129-KO/Xist WT-Cast

chip antibody

N/A

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Nuclei were isolated from formaldehyde crosslinked NSCs and chromatin was fragmented by sonication. Chromatin immunoprecipitation was performed with corresponding antibodies. DNA was extracted from the immunoprecipitated fraction following reverse-crosslinking. Libraries were prepared according to standard Illumina Tru-seq library protocols

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

100093895

Reads aligned (%)

97.6

Duplicates removed (%)

17.6

Number of peaks

270 (qval < 1E-05)

mm9

Number of total reads

100093895

Reads aligned (%)

97.4

Duplicates removed (%)

17.9

Number of peaks

287 (qval < 1E-05)