Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Blood

Cell type

Monocytes

MeSH Description

Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.

Sample

source_name

human periphery blood monocytes

cell type

monocytes

chip antibody

H3K27ac (CST #4353, lot 1)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP DNA was fragmented to 100-500bp by sonication or micrococcal nuclease digestion. DNA-end was repaired to overhang a 3’-dA, then adapters were ligated to the end DNA fragments. DNA fragments with proper size (usually 100-300bp, including adaptor sequence) were selected after PCR amplification. Finally we got qualified library for sequencing.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

25697829

Reads aligned (%)

73.7

Duplicates removed (%)

5.5

Number of peaks

5502 (qval < 1E-05)

hg19

Number of total reads

25697829

Reads aligned (%)

73.1

Duplicates removed (%)

6.6

Number of peaks

5967 (qval < 1E-05)