Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC derived chondrocytes
NA
NA

Attributes by original data submitter

Sample

source_name
Embryonic stem cell-derived chondrocytes - day 60
cell type
Chondrocytes
chip antibody
None

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed with about 10,000 cells per IP using Diagenode Low Cell ChIP kit (cat# C01010072 ) according to manufacturer's protocol. 1 µg of corresponding antibody from Diagenode was used per IP. ChIP-seq libraries wer constructed using Nugen's Ovation Ultralow DR Multiplex System 1-8 (cat# 0330-32) according to manufacturer's instructions. Library quality and concentration was assessed using Agilent Bioanalyzer 2100 and Qubit.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
16787518
Reads aligned (%)
95.7
Duplicates removed (%)
30.8
Number of peaks
193 (qval < 1E-05)

hg19

Number of total reads
16787518
Reads aligned (%)
95.4
Duplicates removed (%)
31.2
Number of peaks
217 (qval < 1E-05)

Base call quality data from DBCLS SRA