GSM3041102: H1-60d rep1 H3K4me3; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K4me3
Cell type
Cell type Class
Pluripotent stem cell
Cell type
hESC derived chondrocytes
NA
NA
Attributes by original data submitter
Sample
source_name
Embryonic stem cell-derived chondrocytes - day 60
cell type
Chondrocytes
chip antibody
H3K4me3, Diagenode, C15410003-50
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed with about 10,000 cells per IP using Diagenode Low Cell ChIP kit (cat# C01010072 ) according to manufacturer's protocol. 1 µg of corresponding antibody from Diagenode was used per IP. ChIP-seq libraries wer constructed using Nugen's Ovation Ultralow DR Multiplex System 1-8 (cat# 0330-32) according to manufacturer's instructions. Library quality and concentration was assessed using Agilent Bioanalyzer 2100 and Qubit.