Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Pluripotent stem cell

Cell type

hESC derived chondrocytes

NA

NA

Sample

source_name

Embryonic stem cell-derived chondrocytes - day 14

cell type

Chondrocytes

chip antibody

H3K4me3, Diagenode, C15410003-50

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP was performed with about 10,000 cells per IP using Diagenode Low Cell ChIP kit (cat# C01010072 ) according to manufacturer's protocol. 1 µg of corresponding antibody from Diagenode was used per IP. ChIP-seq libraries wer constructed using Nugen's Ovation Ultralow DR Multiplex System 1-8 (cat# 0330-32) according to manufacturer's instructions. Library quality and concentration was assessed using Agilent Bioanalyzer 2100 and Qubit.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

17525152

Reads aligned (%)

87.2

Duplicates removed (%)

46.0

Number of peaks

10470 (qval < 1E-05)

hg19

Number of total reads

17525152

Reads aligned (%)

86.5

Duplicates removed (%)

47.2

Number of peaks

10526 (qval < 1E-05)