GSM3041071: Fetal-17w rep1 H3K4me1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K4me1
Cell type
Cell type Class
Bone
Cell type
Knee cartilage
NA
NA
Attributes by original data submitter
Sample
source_name
adult knee cartilage
tissue
knee cartilage
developmental stage
adult
cell type
Chondrocytes
chip antibody
H3K4me1, Diagenode, C15410194
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed with about 10,000 cells per IP using Diagenode Low Cell ChIP kit (cat# C01010072 ) according to manufacturer's protocol. 1 µg of corresponding antibody from Diagenode was used per IP. ChIP-seq libraries wer constructed using Nugen's Ovation Ultralow DR Multiplex System 1-8 (cat# 0330-32) according to manufacturer's instructions. Library quality and concentration was assessed using Agilent Bioanalyzer 2100 and Qubit.