Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
CDK6

Cell type

Cell type Class
Uterus
Cell type
KB
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
ChIP-Seq Analysis of CDK6 in KB Cells
treatment
none
antibody
anti-CDK6 (Santa Cruz, sc-177)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP lysis buffer (1% SDS, 10mM EDTA, 50mM Tris pH 8.1, 1mM PMSF, Roche protease inhibitor mix), followed by IP and purification of DNA Sequencing libraries were prepared from 10 ng of immunoprecipitated DNA with the Illumina ChIPSeq DNA Sample Prep Kit according to Illuminas’ instructions

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

hg19

Number of total reads
40570127
Reads aligned (%)
35.7
Duplicates removed (%)
54.0
Number of peaks
1200 (qval < 1E-05)

hg38

Number of total reads
40570127
Reads aligned (%)
37.3
Duplicates removed (%)
52.4
Number of peaks
1073 (qval < 1E-05)

Base call quality data from DBCLS SRA