Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Neural
Cell type
Neural Stem Cells
MeSH Description
Self-renewing cells that generate the main phenotypes of the nervous system in both the embryo and adult. Neural stem cells are precursors to both NEURONS and NEUROGLIA.

Attributes by original data submitter

Sample

source_name
Neural stem cells
cell type
Neural stem cells
strain
C57BL/6
Sex
female

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Nuclei were isolated from formaldehyde crosslinked NSCs and chromatin was fragmented by either 30 minutes sonication in non-ionic detergent to release the inactive X chromosome or MNase Digestion. Chromatin immunoprecipitation was performed with corresponding antibodies. DNA was extracted from the immunoprecipitated fraction following reverse-crosslinking. ChIP-seq library construction using standard Illumina protocols

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
55491192
Reads aligned (%)
95.7
Duplicates removed (%)
66.3
Number of peaks
741 (qval < 1E-05)

mm9

Number of total reads
55491192
Reads aligned (%)
95.5
Duplicates removed (%)
66.4
Number of peaks
720 (qval < 1E-05)

Base call quality data from DBCLS SRA