Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Digestive tract
Cell type
HCT 116
Primary Tissue
Colon
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
Cell line
cell line
colorectal cancer cell line - HCT116
stale expression
FLAG-ZBTB24
chip antibody
Input

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Genomic DNA was isolated and purified from cells in culture using a serial phenol: chloroform extraction. Trizol reagent was used for extracting total RNA following the manufacturer's protocol. RNA samples are converted to Illumina sequencing libraries using Illumina's Truseq Stranded Total RNA Library Preparation Kit;ChIP-seq library is prepared with ThruPLEX DNA-seq kit.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
16714877
Reads aligned (%)
97.8
Duplicates removed (%)
2.4
Number of peaks
182 (qval < 1E-05)

hg19

Number of total reads
16714877
Reads aligned (%)
97.0
Duplicates removed (%)
2.5
Number of peaks
166 (qval < 1E-05)

Base call quality data from DBCLS SRA