GSM1266817: DMSO SMAD2 ChIP; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
SMAD2
Cell type
Cell type Class
Pluripotent stem cell
Cell type
hESC WA09
NA
NA
Attributes by original data submitter
Sample
source_name
WA09
cell type
Human embryonic stem cells
cell line
WA09
growth conditions
Grown on matrigel/MEF conditioned DMEM/F12, 20% KO serum
treatment
4h DMSO treated
chip antibody
SMAD2 (Cell Signaling)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed for 10min with 1% Formaldehyde, nuclei were extracted (hypotonic lysis), DNA sheared by sonication Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Part# 0801-0303).