Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Embryo
Cell type
Embryonic liver
NA
NA

Attributes by original data submitter

Sample

source_name
liver
strain
CD-1
developmental stage
E14.5
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Tissues were cross-linked, lysed, and sonicated and immunoprecipation was performed using H3K27ac (Abcam Ab4729). Input samples represent sonicated DNA before immunoprecipitation Libraries were constructed using standard protocols and the Illumina TruSeq kit with index barcodes.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
62239630
Reads aligned (%)
87.2
Duplicates removed (%)
11.6
Number of peaks
778 (qval < 1E-05)

mm9

Number of total reads
62239630
Reads aligned (%)
87.0
Duplicates removed (%)
11.5
Number of peaks
875 (qval < 1E-05)

Base call quality data from DBCLS SRA