Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Embryo
Cell type
Embryonic heart
NA
NA

Attributes by original data submitter

Sample

source_name
heart
strain
CD-1
developmental stage
E17.5
chip antibody
H3K27ac (Abcam 4729)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Tissues were cross-linked, lysed, and sonicated and immunoprecipation was performed using H3K27ac (Abcam Ab4729). Input samples represent sonicated DNA before immunoprecipitation Libraries were constructed using standard protocols and the Illumina TruSeq kit with index barcodes.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
47996059
Reads aligned (%)
96.0
Duplicates removed (%)
7.5
Number of peaks
19644 (qval < 1E-05)

mm9

Number of total reads
47996059
Reads aligned (%)
95.9
Duplicates removed (%)
7.5
Number of peaks
19677 (qval < 1E-05)

Base call quality data from DBCLS SRA