Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Cebpa

Cell type

Cell type Class
Blood
Cell type
B cells
NA
NA

Attributes by original data submitter

Sample

source_name
primary Bcell after 18h treatment of estradiol
cell type
primary B cells
treatment
Estradiol for 18h
chip antibody
anti-Cebpa (Santa Cruz, catalog: sc-61, lot: k1009)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP experiments were performed as described previously (van Oevelen et al, 2008, PMID:20956564). DNA libraries of C/EBPa enriched chromatin fragments were prepared using Illumina's reagents and instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
29435701
Reads aligned (%)
93.6
Duplicates removed (%)
27.6
Number of peaks
565 (qval < 1E-05)

mm9

Number of total reads
29435701
Reads aligned (%)
93.2
Duplicates removed (%)
27.5
Number of peaks
606 (qval < 1E-05)

Base call quality data from DBCLS SRA