Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MLLT3

Cell type

Cell type Class
Blood
Cell type
CD34 Hematopoietic stem cells
NA
NA

Attributes by original data submitter

Sample

source_name
human FL CD34+CD38-/lowCD90+GFP+
cell type
4w cultured HSPC
genotype/variation
MLLT3 overexpression
chip antibody
Genetex, GTX102835

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells are crosslinked 10' in 1%formaldehide, lysed and sonicated. The indicated mark is immunoprecipitated overnaight, and enriched by magnetical isolation. IP fraction is eluted and decrosslinked. Ovation Ultralow v2 (Nugen)

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg19

Number of total reads
19571657
Reads aligned (%)
55.7
Duplicates removed (%)
36.3
Number of peaks
1161 (qval < 1E-05)

hg38

Number of total reads
19571657
Reads aligned (%)
57.4
Duplicates removed (%)
35.0
Number of peaks
1118 (qval < 1E-05)

Base call quality data from DBCLS SRA