Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Epitope tags

Cell type

Cell type Class
Blood
Cell type
CD34 Hematopoietic stem cells
NA
NA

Attributes by original data submitter

Sample

source_name
human FL CD34+CD38-/lowCD90+GFP+
cell type
4w cultured HSPC
genotype/variation
control
chip antibody
Abcam, ab15828

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells are crosslinked 10' in 1%formaldehide, lysed and sonicated. The indicated mark is immunoprecipitated overnaight, and enriched by magnetical isolation. IP fraction is eluted and decrosslinked. Ovation Ultralow v2 (Nugen)

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg38

Number of total reads
14033466
Reads aligned (%)
94.8
Duplicates removed (%)
31.3
Number of peaks
879 (qval < 1E-05)

hg19

Number of total reads
14033466
Reads aligned (%)
93.5
Duplicates removed (%)
33.5
Number of peaks
883 (qval < 1E-05)

Base call quality data from DBCLS SRA