GSM3031505: K562 AID-BRD4 input DMSO; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous
Attributes by original data submitter
Sample
source_name
K562_AID-BRD4_input_DMSO
treatment
DMSO (1:5000), 60 min
cell line
K562
genetic modification
AID-BRD4 / SFFV-OsTir1-T2A-BlastR
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were cross-linked with 1% formaldehyde (10', RT). Nuclei were isolated, lysed, and chromatin was sheared by sonication. Mouse chromatin (ratio 10:1, human:mouse) was added for internal normalization. Antibodies against total Pol2, Pol2-S2p or Pol2-S5p (active motif) were added overnight at 4° and captured with magnetic sepharose beads for 2h at 4°C. Beads were washed, immune complexes eluted, and cross-links reversed for 6h at 65°C. Genomic DNA was recovered by phenol-chloroform extraction and ethanol precipitation. Deep sequencing libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina.