Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pancreas
Cell type
Pancreas
MeSH Description
A nodular organ in the ABDOMEN that contains a mixture of ENDOCRINE GLANDS and EXOCRINE GLANDS. The small endocrine portion consists of the ISLETS OF LANGERHANS secreting a number of hormones into the blood stream. The large exocrine portion (EXOCRINE PANCREAS) is a compound acinar gland that secretes several digestive enzymes into the pancreatic ductal system that empties into the DUODENUM.

Attributes by original data submitter

Sample

source_name
Pancreas
tissue
Pancreas
strains
mixed (129X1/Sv / C57BL/6)
antibody
none
genotype/variation
C-OSKM
treatment
Dox3days2W

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Purified ChIP DNA (5 ng per sample) was used for generation of sequencing libraries with TruSeq ChIP Sample Prep Kit (Illumina). The resultant DNA libraries were assessed on an Agilent Bioanalyzer and quantified with KAPA Library Quantification Kits (KAPA BIOSYSTEMS). The libraries were sequenced to generate single-end 86 bp reads using NextSeq 500 (illumina).

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
27750840
Reads aligned (%)
98.3
Duplicates removed (%)
18.6
Number of peaks
253 (qval < 1E-05)

mm9

Number of total reads
27750840
Reads aligned (%)
98.0
Duplicates removed (%)
18.6
Number of peaks
218 (qval < 1E-05)

Base call quality data from DBCLS SRA