Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K9ac

Cell type

Cell type Class
Blood
Cell type
U-937
Primary Tissue
Blood
Tissue Diagnosis
Lymphoma Diffuse Large B Cell

Attributes by original data submitter

Sample

source_name
U-937 cells (monocytes)
cell line
U-937
treatment
untreated control
time point
96 hours after seed-out
chip antibody
anti H3K9ac, Active Motif, 39917, LOT: 23913004

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Nuclei were extracted by sonication with low power, separated by centrifugation, lysed and chromatin was sheared by sonication with high power. Histone-DNA complexes were immunoprecipitated. Libraries were constructed using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB), following the manufacturer's instructions

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
18137053
Reads aligned (%)
97.9
Duplicates removed (%)
33.1
Number of peaks
15071 (qval < 1E-05)

hg19

Number of total reads
18137053
Reads aligned (%)
97.4
Duplicates removed (%)
33.9
Number of peaks
15166 (qval < 1E-05)

Base call quality data from DBCLS SRA