Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
U-937
Primary Tissue
Blood
Tissue Diagnosis
Lymphoma Diffuse Large B Cell

Attributes by original data submitter

Sample

source_name
U-937 cells (monocytes)
cell line
U-937
treatment
decitabine + entinostat
time point
96 hours after seed-out
chip antibody
anti H3K27ac, Active Motif, 39133, LOT: 31814008

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Nuclei were extracted by sonication with low power, separated by centrifugation, lysed and chromatin was sheared by sonication with high power. Histone-DNA complexes were immunoprecipitated. Libraries were constructed using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB), following the manufacturer's instructions

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
61163216
Reads aligned (%)
99.0
Duplicates removed (%)
4.0
Number of peaks
803 (qval < 1E-05)

hg19

Number of total reads
61163216
Reads aligned (%)
98.6
Duplicates removed (%)
4.6
Number of peaks
921 (qval < 1E-05)

Base call quality data from DBCLS SRA