GSM1258439: TX1072 ES Input; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
Embryonic stem cells
genotype/variation
TX1072
dox treatment
no
cell passage
5
antibody
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and JARID2-DNA complexes were isolated with JARID2 antibody (Li et al., 2010) ChIP-seq libraries were prepared according to Illumina protocol and sequenced using a HiSeq machine. Single-end sequencing was carried out to obtain about 50 million (M), 100bp long reads per sample. Read alignment with Bowtie2-2.1.0 (Langmead and Salzberg, 2012; Langmead et al., 2009) with standard parameters and one mismatch in the seed sequence and consecutive duplicate filtering resulted in at least 35M reads per sample. Only reads with mapping quality Qphred20 were kept for the enrichment analysis.