Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Muscle
Cell type
C2C12
Primary Tissue
Skeletal Muscle
Tissue Diagnosis
NOS

Attributes by original data submitter

Sample

source_name
C2C12
treatment 1
DMEM containing 2% donor horse serum (EuroClone)_ 24 hours (day1)
chip antibody
none
cell type
myoblasts

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For DNA purification, the QIAquick PCR purification kit was used (Qiagen). DNA was further sonicated after elution, using the Ultra-Sonicator E220 (CovarisTM) at 10 V for 90 seconds, and the fragments sizes were evaluated by the Bioanalyzer (Agilent Technologies) Accel-NGS 2S Plus DNA Library Kit (Swift Biosciences)

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
24100271
Reads aligned (%)
98.8
Duplicates removed (%)
11.7
Number of peaks
144 (qval < 1E-05)

mm9

Number of total reads
24100271
Reads aligned (%)
98.8
Duplicates removed (%)
11.7
Number of peaks
135 (qval < 1E-05)

Base call quality data from DBCLS SRA