For DNA purification, the QIAquick PCR purification kit was used (Qiagen). DNA was further sonicated after elution, using the Ultra-Sonicator E220 (CovarisTM) at 10 V for 90 seconds, and the fragments sizes were evaluated by the Bioanalyzer (Agilent Technologies) Accel-NGS 2S Plus DNA Library Kit (Swift Biosciences)