SRX3729397
GSM3018470: PUI1 SenDMSO ChIP-seq; Mus musculus; ChIP-Seq

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Spi1

Cell type

Cell type Class
Blood
Cell type
Hematopoietic progenitors
NA
NA

Attributes by original data submitter

Sample

source_name
MLL-AF9 I-BET sensitive cells
tissue
haematopoietic progenitors
condition
I-BET Sensitive
strain
C57BL/6
chip antibody
anti-PU.1/SPI1 (sc325, Santa Cruz Biotechnology)
treatment
DMSO

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysate were isolated from formaldehybe cross-linked cells and sonicated to release the chromatin, then immunoprecipitated with antibody ThruPLEXTM-FD Prep Kit protocol (Rubicon Genomics)

Sequencing Platform

instrument_model
NextSeq 500

Where can I get the processing logs?

Read processing pipeline log

mm10

Number of total reads
9471840
Reads aligned (%)
94.4
Duplicates removed (%)
16.1
Number of peaks
19553 (qval < 1E-05)

mm9

Number of total reads
9471840
Reads aligned (%)
94.3
Duplicates removed (%)
16.2
Number of peaks
19524 (qval < 1E-05)

Base call quality data from DBCLS SRA