Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Blood

Cell type

Hematopoietic progenitors

NA

NA

Sample

source_name

MLL-AF9 I-BET resistant cells

tissue

haematopoietic progenitors

condition

I-BET Resistant

strain

C57BL/6

chip antibody

anti-H3K27ac (ab4729, Abcam)

treatment

IBET

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysate were isolated from formaldehybe cross-linked cells and sonicated to release the chromatin, then immunoprecipitated with antibody ThruPLEXTM-FD Prep Kit protocol (Rubicon Genomics)

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

10367275

Reads aligned (%)

97.8

Duplicates removed (%)

8.4

Number of peaks

26484 (qval < 1E-05)

mm9

Number of total reads

10367275

Reads aligned (%)

97.7

Duplicates removed (%)

8.5

Number of peaks

26479 (qval < 1E-05)