Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
Hematopoietic progenitors
NA
NA

Attributes by original data submitter

Sample

source_name
MLL-AF9 I-BET sensitive cells
tissue
haematopoietic progenitors
condition
I-BET Sensitive
strain
C57BL/6
chip antibody
anti-H3K27ac (ab4729, Abcam)
treatment
DMSO

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysate were isolated from formaldehybe cross-linked cells and sonicated to release the chromatin, then immunoprecipitated with antibody ThruPLEXTM-FD Prep Kit protocol (Rubicon Genomics)

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
10194722
Reads aligned (%)
98.0
Duplicates removed (%)
9.3
Number of peaks
25797 (qval < 1E-05)

mm9

Number of total reads
10194722
Reads aligned (%)
97.9
Duplicates removed (%)
9.4
Number of peaks
25797 (qval < 1E-05)

Base call quality data from DBCLS SRA