Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
GATA6

Cell type

Cell type Class
Digestive tract
Cell type
HuG1-N
Primary Tissue
Stomach
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
HUG1N_GATA6_ChIP
cell line
HuG-1
cell type
gastric carcinoma
chip antibody
GATA6
chip antibody vendor
Cell Signaling

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were lysed in SDS lysis buffer. Cells were crosslinked at 50% confluency with 1% formaldehyde. Libraries were made using the NEB kit following the instructions of the manufacturer.

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

hg19

Number of total reads
24838312
Reads aligned (%)
63.2
Duplicates removed (%)
5.2
Number of peaks
2309 (qval < 1E-05)

hg38

Number of total reads
24838312
Reads aligned (%)
64.3
Duplicates removed (%)
4.2
Number of peaks
2201 (qval < 1E-05)

Base call quality data from DBCLS SRA