Antigen

Antigen Class

DNase-seq

Antigen

DNase-Seq

Cell type

Cell type Class

Prostate

Cell type

LNCAP

Primary Tissue

Prostate

Tissue Diagnosis

Carcinoma

Sample

source_name

Prostate cancer cell line LNCaP

cell line

LNCaP

passage

14-17

digestion level

50U

fragment size

200-300bp

Sequenced DNA Library

library_strategy

DNase-Hypersensitivity

library_source

GENOMIC

library_selection

DNase

library_construction_protocol

LNCaP and MCF-7 cells were harvested by trypsinization followed by nuclei extraction. Entact nuclei were treated with Dnase I for desired time. Libraries were prepared according to Rubicon's instructions accompanying the ThruPLEX-FD kit.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

78233912

Reads aligned (%)

98.5

Duplicates removed (%)

31.5

Number of peaks

60329 (qval < 1E-05)

hg19

Number of total reads

78233912

Reads aligned (%)

97.8

Duplicates removed (%)

32.9

Number of peaks

59999 (qval < 1E-05)