Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562
chip antibody
-
cell description
Haematopoietic and lymphoid
cell line
K562

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with IgG antibody. Libraries were prepared for sequencing using standard Illumina protocols with modifications; Reference paper:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3813302/

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
13440685
Reads aligned (%)
96.4
Duplicates removed (%)
6.3
Number of peaks
208 (qval < 1E-05)

hg19

Number of total reads
13440685
Reads aligned (%)
95.4
Duplicates removed (%)
6.5
Number of peaks
184 (qval < 1E-05)

Base call quality data from DBCLS SRA