Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pancreas
Cell type
Islets of Langerhans
MeSH Description
Irregular microscopic structures consisting of cords of endocrine cells that are scattered throughout the PANCREAS among the exocrine acini. Each islet is surrounded by connective tissue fibers and penetrated by a network of capillaries. There are four major cell types. The most abundant beta cells (50-80%) secrete INSULIN. Alpha cells (5-20%) secrete GLUCAGON. PP cells (10-35%) secrete PANCREATIC POLYPEPTIDE. Delta cells (~5%) secrete SOMATOSTATIN.

Attributes by original data submitter

Sample

source_name
whole islet
strain
C57B6/J
age
8-10 weeks
genotype
wild type
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
NEXSON ChIP-seq workflow (Arrigoni et al., 2015) was applied to freshly isolated mouse islets Primary islets were obtained by collagenase digestion with reference to (Li et al., 2009). In brief, adult pancreata were perfused through the common bile duct using a 27/30-gauge needle with Collegenase IV (Sigma) solution. Perfused pancreata were digested and purified by centrifugation in Histopaque gradient (Sigma). Isolated islets were hand picked and cultured in complete media (RMPI-1640 containing 11mM glucose, 10% FBS, 0.1% Penincillin/Streptomycin) and maintained at 37°C in 5% CO2 environment.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
44175748
Reads aligned (%)
96.5
Duplicates removed (%)
13.3
Number of peaks
207 (qval < 1E-05)

mm9

Number of total reads
44175748
Reads aligned (%)
96.3
Duplicates removed (%)
14.1
Number of peaks
202 (qval < 1E-05)

Base call quality data from DBCLS SRA