Antigen

Antigen Class

TFs and others

Antigen

HDAC2

Cell type

Cell type Class

Blood

Cell type

HL-60

Primary Tissue

Blood

Tissue Diagnosis

Leukemia

Sample

source_name

HL-60 cell

cell type

Human promyelocytic leukemia cells

treatment

DMSO

passages

80-85

chip antibody

HDAC2 (Abcam [ab12169])

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. The construction of library was performed using TruSeq ChIP Library Preparation Kits according to the manufacturer's instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

9633236

Reads aligned (%)

95.2

Duplicates removed (%)

6.1

Number of peaks

365 (qval < 1E-05)

hg19

Number of total reads

9633236

Reads aligned (%)

94.2

Duplicates removed (%)

6.3

Number of peaks

322 (qval < 1E-05)