Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
HDAC2

Cell type

Cell type Class
Blood
Cell type
HL-60
Primary Tissue
Blood
Tissue Diagnosis
Leukemia

Attributes by original data submitter

Sample

source_name
HL-60 cell
cell type
Human promyelocytic leukemia cells
treatment
DMSO
passages
80-85
chip antibody
HDAC2 (Abcam [ab12169])

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. The construction of library was performed using TruSeq ChIP Library Preparation Kits according to the manufacturer's instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
9633236
Reads aligned (%)
176.6
Duplicates removed (%)
6.3
Number of peaks
322 (qval < 1E-05)

hg38

Number of total reads
9633236
Reads aligned (%)
178.9
Duplicates removed (%)
6.1
Number of peaks
365 (qval < 1E-05)

Base call quality data from DBCLS SRA