Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Blood

Cell type

CUTLL1

NA

NA

Sample

source_name

CUTLL1

cell line

CUTLL1

tumor stage

T lymphoblastic leukemia/lymphoma cell line

cell type

T lymphocyte

treatment

GSI 72 hrs

antibody

H3K27ac (Abcam ab4729)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. Libraries were prepared according to New England Biolabs instructions accompanying the DNA Sample Prep Master Mix Set 1 (NEB E6040S).

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

59343434

Reads aligned (%)

84.4

Duplicates removed (%)

23.7

Number of peaks

41485 (qval < 1E-05)

hg19

Number of total reads

59343434

Reads aligned (%)

84.2

Duplicates removed (%)

23.8

Number of peaks

41480 (qval < 1E-05)