Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Bone
Cell type
U2OS
Tissue
bone
Lineage
mesoderm
Description
osteosarcoma from the tibia of a 15 year old, J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma, viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses, mycoplasma contamination was detected and eliminated in 1972, (PMID: 6081590)

Attributes by original data submitter

Sample

name
SET8_12h_input_gammaH2AX
ChIP_antibody
no
time_after_thymidine_release(h)
12
cel_line
U20S
RNAi_target
SET8

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
16765220
Reads aligned (%)
99.2
Duplicates removed (%)
7.3
Number of peaks
663 (qval < 1E-05)

hg19

Number of total reads
16765220
Reads aligned (%)
98.2
Duplicates removed (%)
8.9
Number of peaks
788 (qval < 1E-05)

Base call quality data from DBCLS SRA